Browse
Submit Data
Databases
API
Help

Dataset Information

7 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chip-based ion chromatography (chip-IC) with a sensitive five-electrode conductivity detector for the simultaneous detection of multiple ions in drinking water.

ABSTRACT: The emerging need for accurate, efficient, inexpensive, and multiparameter monitoring of water quality has led to interest in the miniaturization of benchtop chromatography systems. This paper reports a chip-based ion chromatography (chip-IC) system in which the microvalves, sample channel, packed column, and conductivity detector are all integrated on a polymethylmethacrylate (PMMA) chip. A laser-based bonding technique was developed to guarantee simultaneous robust sealing between the homogeneous and heterogeneous interfaces. A five-electrode-based conductivity detector was presented to improve the sensitivity for nonsuppressed anion detection. Common anions (F^-, Cl^-, NO₃ ^-, and SO₄ ^2-) were separated in less than 8 min, and a detection limit (LOD) of 0.6 mg L^-1 was achieved for SO₄ ^2-. Tap water was also analyzed using the proposed chip-IC system, and the relative deviations of the quantified concentration were less than 10% when compared with that a commercial IC system.

SUBMITTER: Li X

PROVIDER: S-EPMC8433475 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

On-Chip Cyclic Voltammetry Measurements Using a Compact 1024-Electrode CMOS IC.

Project description:Complementary metal-oxide-semiconductor (CMOS) microelectrode arrays integrate amplifier arrays with on-chip electrodes, offering high-throughput platforms for electrochemical sensing with high spatial and temporal resolution. Such devices have been developed for highly parallel constant voltage amperometric detection of transmitter release from multiple cells with single-vesicle resolution. Cyclic voltammetry (CV) is an electrochemical method that applies voltage waveforms, which provides additional information about electrode properties and about the nature of analytes. A 16-channel, 64-electrode-per-channel CMOS integrated circuit (IC) fabricated in a 0.5 μm CMOS process for CV is demonstrated. Each detector consists of only 11 transistors and an integration capacitor with a unit dimension of 0.0015 mm2. The device was postfabricated using Pt as the working electrode material with a shifted electrode design, which makes it possible to redefine the size and the location of working electrodes. The system incorporating cell-sized (8 μm radius) microelectrodes was validated with dopamine injection tests and CV measurements of potassium ferricyanide at a 1 V/s scanning rate. The cyclic voltammograms were in excellent agreement with theoretical predictions. The technology enables rigorous characterization of electrode performance for the application of CMOS microelectrode arrays in low-noise amperometric measurements of quantal transmitter release as well as other biosensing applications.

| S-EPMC8650766 | biostudies-literature

Dual-Purpose Photometric-Conductivity Detector for Simultaneous and Sequential Measurements in Flow Analysis.

Project description:This work presents a new dual-purpose detector for photometric and conductivity measurements in flow-based analysis. The photometric detector is a paired emitter-detector diode (PEDD) device, whilst the conductivity detection employs a capacitively coupled contactless conductivity detector (C4D). The flow-through detection cell is a rectangular acrylic block (ca. 2 × 2 × 1.5 cm) with cylindrical channels in Z-configuration. For the PEDD detector, the LED light source and detector are installed inside the acrylic block. The two electrodes of the C4D are silver conducting ink painted on the PEEK inlet and outlet tubing of the Z-flow cell. The dual-purpose detector is coupled with a sequential injection analysis (SIA) system for simultaneous detection of the absorbance of the orange dye and conductivity of the dissolved oral rehydration salt powder. The detector was also used for sequential measurements of creatinine and the conductivity of human urine samples. The creatinine analysis is based on colorimetric detection of the Jaffé reaction using the PEDD detector, and the conductivity of the urine, as measured by the C4D detector, is expressed in millisiemens (mS cm-1).

| S-EPMC7287826 | biostudies-literature

EWOD Chip with Micro-Barrier Electrode for Simultaneous Enhanced Mixing during Transportation.

Project description:Digital microfluidic platforms have been extensively studied in biology. However, achieving efficient mixing of macromolecules in microscale, low Reynolds number fluids remains a major challenge. To address this challenge, this study presents a novel design solution based on dielectric electro-wetting (EWOD) by optimizing the geometry of the transport electrode. The new design integrates micro-barriers on the electrodes to generate vortex currents that promote mixing during droplet transport. This design solution requires only two activation signals, minimizing the number of pins required. The mixing performance of the new design was evaluated by analyzing the degree of mixing inside the droplet and quantifying the motion of the internal particles. In addition, the rapid mixing capability of the new platform was demonstrated by successfully mixing the sorbitol solution with the detection solution and detecting the resulting reaction products. The experimental results show that the transfer electrode with a micro-barrier enables rapid mixing of liquids with a six-fold increase in mixing efficiency, making it ideal for the development of EWOD devices.

| S-EPMC10459807 | biostudies-literature

New Analytical Method for Measuring the Atomic Weight of Neon Using Gas Chromatography with a Thermal Conductivity Detector.

Project description:The atomic weights of neon (Ne) gases were measured by gas chromatography with a thermal conductivity detector (GC-TCD). High-purity neon gas was used as the carrier and sample gases in this study, which is different from typical GC analysis. The peak signals from the GC-TCD appear when the thermal conductivity between the sample and carrier gases is different. In most gaseous molecules, the thermal conductivity has been assumed to be the same if the chemical species is the same. However, the thermal conductivity of neon gases shows different values among several manufacturers, because the relative abundance of the 22Ne isotope, which is quite large (∼10% in atmospheric neon), varies due to the mass fractionation during air separation. We identified the atomic weights of seven neon gases. Additionally, the absolute isotope ratios of all neon gases were measured using a magnetic sector type gas/mass spectrometer. The atomic weights of the seven neon gases were compared with the results obtained from GC-TCD, and the results agreed with each other within the expanded uncertainty (k = 2).

| S-EPMC8190878 | biostudies-literature

Evaluating gas chromatography with a halogen-specific detector for the determination of disinfection by-products in drinking water.

Project description:The occurrence of disinfection by-products (DBPs) in drinking water has become an issue of concern during the past decades. The DBPs pose health risks and are suspected to cause various cancer forms, be genotoxic, and have negative developmental effects. The vast chemical diversity of DBPs makes comprehensive monitoring challenging. Only few of the DBPs are regulated and included in analytical protocols. In this study, a method for simultaneous measurement of 20 DBPs from five different structural classes (both regulated and non-regulated) was investigated and further developed for 11 DBPs using solid-phase extraction and gas chromatography coupled with a halogen-specific detector (XSD). The XSD was highly selective towards halogenated DBPs, providing chromatograms with little noise. The method allowed detection down to 0.05 μg L-1 and showed promising results for the simultaneous determination of a range of neutral DBP classes. Compounds from two classes of emerging DBPs, more cytotoxic than the "traditional" regulated DBPs, were successfully determined using this method. However, haloacetic acids (HAAs) should be analyzed separately as some HAA methyl esters may degrade giving false positives of trihalomethanes (THMs). The method was tested on real water samples from two municipal waterworks where the target DBP concentrations were found below the regulatory limits of Sweden.

| S-EPMC6447507 | biostudies-literature

Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis.

Project description:In this work, we report a fast, portable, and economical microfluidic platform for the simultaneous detection of nucleic acid and proteins. Using SARS-CoV-2 as a target, this microfluidic chip enabled to simultaneously detect the SARS-CoV-2 RNA (N gene) antigen (or specific IgG antibody) with respective detection limits of 1 copy/μL for nucleic acid, 0.85 ng/mL for antigen, and 5.80 ng/mL for IgG within 30 min with high stability and anti-interference ability. The capability of this system in clinical applications was further evaluated using clinical samples, displaying 100% sensitivity and 100% specificity for COVID-19 diagnosis. These findings demonstrate the potential of this method to be used for the detection and subsequent control of pathogens.

| S-EPMC9631717 | biostudies-literature

Simultaneous Analysis of Monovalent Anions and Cations with a Sub-Microliter Dead-Volume Flow-Through Potentiometric Detector for Ion Chromatography.

Project description:A microliter dead-volume flow-through cell as a potentiometric detector is described in this article for sensitive, selective and simultaneous detection of common monovalent anions and cations in single column ion chromatography for the first time. The detection cell consisted of less selective anion- and cation-selective composite membrane electrodes together with a solid-state composite matrix reference electrode. The simultaneous separation and sensitive detection of sodium (Na(+)), potassium (K(+)), ammonium (NH4 (+)), chloride (Cl(-)) and nitrate (NO3 (-)) in a single run was achieved by using 98% 1.5 mM MgSO4 and 2% acetonitrile eluent with a mixed-bed ion-exchange separation column without suppressor column system. The separation and simultaneous detection of the anions and cations were completed in 6 min at the eluent flow-rate of 0.8 mL/min. Detection limits, at S/N = 3, were ranged from 0.2 to 1.0 µM for the anions and 0.3 to 3.0 µM for the cations, respectively. The developed method was successfully applied to the simultaneous determination of monovalent anions and cations in several environmental and biological samples.

| S-EPMC4885397 | biostudies-literature

Cheap and sensitive polymer/bismuth film modified electrode for simultaneous determination of Pb(II) and Cd(II) ions.

Project description:Different aminonaphetalenesulphonic acid derivatives like 5-aminonaphthalene-1-sulphonic acid (5AN1SA), 2-aminonaphthalene-1-sulphonic acid (2AN1SA), 8-aminonaphthalene-2-sulphonic acid (8AN2SA) and 4-amino-3-hydroxynaphthalene-1-sulphonic acid (4A3HN1SA) were used to construct polymer/bismuth film modified electrode for simultaneous determination of Pb(II) and Cd(II) ions with the aim of developing a cheaper and sensitive electrode that could possibly replace Nafion. Among the different modified electrodes, poly (8AN2SA)/bismuth film modified electrodes showed the highest electrochemical response for both ions. These electrochemical results were also supported by density functional theory (DFT) calculations. Based on these experimental and theoretical results, poly (8AN2SA)/bismuth film glassy carbon modified electrode was further investigated to develop a simple and sensitive electrochemical method for the simultaneous determination of Pb(II) and Cd(II) ions. After optimizing the different experimental parameters, the proposed method gave a linear range of 1-40 μg/L with the detection limit of 0.38 and 0.08 μg/L for the simultaneous determination of Pb(II) and Cd(II) ions, respectively.

| S-EPMC8648549 | biostudies-literature

Broadband Laser-Based Infrared Detector for Gas Chromatography.

Project description:Cantilever-enhanced photoacoustic spectroscopy coupled with gas chromatography is used to quantitatively analyze a mixture of alcohols in a quasi-online manner. A full identification and quantification of all analytes are achieved based on their spectral fingerprints using a widely tunable continuous-wave laser as a light source. This can be done even in the case of interfering column/septum bleed or simultaneously eluted peaks. The combination of photoacoustic spectroscopy and gas chromatography offers a viable solution for compact and portable instruments in applications that require straightforward analyses with no consumables.

| S-EPMC7660589 | biostudies-literature

Simultaneous Analysis of Fenthion and Its Five Metabolites in Produce Using Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry.

Project description:A simultaneous analytical method for the organophosphorus insecticide fenthion and its five metabolites (fenthion oxon, fenthion oxon sulfoxide, fenthion oxon sulfone, fenthion sulfoxide, and fenthion sulfone) was developed based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Five matrices (brown rice, chili pepper, orange, potato, and soybean) were selected to validate the method. The target compounds were analyzed using positive electrospray ionization in the multiple reaction monitoring mode. For the best sensitivity in regard to the detector response, water and methanol containing formic acid (0.1%) were selected as the mobile phase. The optimum extraction efficiency was obtained through a citrate-buffered QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. Recovery tests were carried out at three spiking levels (n = 3). At all fortification levels, the accuracy and precision results were between 70% and 120% with a relative standard deviation of ?15%. The limit of quantitation was 0.01 mg/kg, and the correlation coefficients (r2) of the matrix-matched calibration curves were >0.99. Significant signal suppression in the detector responses were observed for all matrices, suggesting that a compensation method, such as matrix-matched calibration, is required to provide accurate quantitative results. The applicability of the presented method was confirmed for the simultaneous analysis of fenthion and its metabolites in various crops.

| S-EPMC7221716 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data