PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes.
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ABSTRACT:
SUBMITTER: Stubbs SL
PROVIDER: S-EPMC84342 | biostudies-literature | 1999 Feb
REPOSITORIES: biostudies-literature
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PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes.
Stubbs S L SL Brazier J S JS O'Neill G L GL Duerden B I BI
Journal of clinical microbiology 19990201 2
A reference library of types of Clostridium difficile has been constructed by PCR ribotyping isolates (n = 2,030) from environmental (n = 89), hospital (n = 1,386), community practitioner (n = 395), veterinary (n = 27), and reference (n = 133) sources. The library consists of 116 distinct types identified on the basis of differences in profiles generated with PCR primers designed to amplify the 16S-23S rRNA gene intergenic spacer region. Isolates from 55% of infections in hospitals in the United ...[more]
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