LncRNA LINC00963 promotes colorectal cancer cell proliferation and metastasis by regulating miR‑1281 and TRIM65.

ABSTRACT: Reportedly, long‑chain non‑coding RNA LINC00963 features prominently in cancer biology. However, functional details of LINC00963 in colorectal cancer (CRC) remain to be elucidated. Reverse transcription‑quantitative (RT‑q)PCR was performed to examine LINC00963 and microRNA (miR)‑1281 expression levels in 53 matched pairs of cancerous and non‑cancerous tissues from patients with CRC. Tripartite motif‑containing 65 (TRIM65) protein expression in CRC cells was detected via western blot analysis. Furthermore, LINC00963 overexpression plasmid, LINC00963 small interfering RNA, miR‑1281 mimics or miR‑1281 inhibitors were transfected into CRC cells, and Cell Counting Kit‑8, colony formation and Transwell assays were adopted to study the effects of LINC00963 and miR‑1281 on the malignant phenotypes

SUBMITTER: Lv H 

PROVIDER: S-EPMC8436205 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature