Unknown

Dataset Information

0

Colorimetric Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of SARS-CoV-2.


ABSTRACT: Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been spreading rapidly all over the world. In the absence of effective treatments or a vaccine, there is an urgent need to develop a more rapid and simple detection technology of COVID-19. We describe a WarmStart colorimetric reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARS-CoV-2. The detection limit for this assay was 1 copy/µL SARS-CoV-2. To test the clinical sensitivity and specificity of the assay, 37 positive and 20 negative samples were used. The WarmStart colorimetric RT-LAMP had 100% sensitivity and specificity. End products were detected by direct observation, thereby eliminating the need for post-amplification processing steps. WarmStart colorimetric RT-LAMP provides an opportunity to facilitate virus detection in resource-limited settings without a sophisticated diagnostic infrastructure.

SUBMITTER: Lai MY 

PROVIDER: S-EPMC8437165 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8276080 | biostudies-literature
| S-EPMC8008787 | biostudies-literature
| S-EPMC7275676 | biostudies-literature
| S-EPMC8688429 | biostudies-literature
| S-EPMC8023013 | biostudies-literature
| S-EPMC7144850 | biostudies-literature
| S-EPMC7216271 | biostudies-literature
| S-EPMC7703389 | biostudies-literature
| S-EPMC3599620 | biostudies-literature
| S-EPMC7656183 | biostudies-literature