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Optogenetic Control of Non-Apoptotic Cell Death.

ABSTRACT: Herein, a set of optogenetic tools (designated LiPOP) that enable photoswitchable necroptosis and pyroptosis in live cells with varying kinetics, is introduced. The LiPOP tools allow reconstruction of the key molecular steps involved in these two non-apoptotic cell death pathways by harnessing the power of light. Further, the use of LiPOPs coupled with upconversion nanoparticles or bioluminescence is demonstrated to achieve wireless optogenetic or chemo-optogenetic killing of cancer cells in multiple mouse tumor models. LiPOPs can trigger necroptotic and pyroptotic cell death in cultured prokaryotic or eukaryotic cells and in living animals, and set the stage for studying the role of non-apoptotic cell death pathways during microbial infection and anti-tumor immunity.

SUBMITTER: He L

PROVIDER: S-EPMC8438606 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Optogenetic Control of Non-Apoptotic Cell Death.

He Lian L Huang Zixian Z Huang Kai K Chen Rui R Nguyen Nhung T NT Wang Rui R Cai Xiaoli X Huang Zhiquan Z Siwko Stefan S Walker Joel R JR Han Gang G Zhou Yubin Y Jing Ji J

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20210506 13

Herein, a set of optogenetic tools (designated LiPOP) that enable photoswitchable necroptosis and pyroptosis in live cells with varying kinetics, is introduced. The LiPOP tools allow reconstruction of the key molecular steps involved in these two non-apoptotic cell death pathways by harnessing the power of light. Further, the use of LiPOPs coupled with upconversion nanoparticles or bioluminescence is demonstrated to achieve wireless optogenetic or chemo-optogenetic killing of cancer cells in mul ...[more]

PMID: 34540558

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