A gain series method for accurate EMCCD calibration.
Ontology highlight
ABSTRACT:
SUBMITTER: Ryan DP
PROVIDER: S-EPMC8443689 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
A gain series method for accurate EMCCD calibration.
Ryan Duncan P DP Dunlap Megan K MK Gelfand Martin P MP Werner James H JH Van Orden Alan K AK Goodwin Peter M PM
Scientific reports 20210915 1
Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other me ...[more]
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