A gain series method for accurate EMCCD calibration.
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ABSTRACT: Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.
SUBMITTER: Ryan DP
PROVIDER: S-EPMC8443689 | biostudies-literature |
REPOSITORIES: biostudies-literature
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