Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs.

ABSTRACT: Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA_A antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.

SUBMITTER: Grupi A 

PROVIDER: S-EPMC8448295 | biostudies-literature |

REPOSITORIES: biostudies-literature