Protocol for evaluating protein relocalization from the plasma membrane to chloroplasts.
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ABSTRACT: We present a protocol for analyzing and evaluating the relocalization of proteins from the plasma membrane to chloroplasts. Some plant membrane-bound proteins carry dual targeting signals, e.g., a membrane-anchoring N-myristoylation motif and a chloroplast transit peptide. These proteins are predominantly targeted to membranes; upon certain cues, however, they can undergo detachment from membranes and relocalization to chloroplasts. This protocol combines imaging and biochemical analyses to track in a reliable and quantitative manner the relocalization of proteins between subcellular organelles. For complete details on the use and execution of this protocol, please refer to Medina-Puche et al. (2020).
SUBMITTER: Medina-Puche L
PROVIDER: S-EPMC8450296 | biostudies-literature |
REPOSITORIES: biostudies-literature
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