Ontology highlight
ABSTRACT:
SUBMITTER: Komuczki D
PROVIDER: S-EPMC8451748 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Komuczki Daniel D Dutra Gregory G Gstöttner Christoph C Dominguez-Vega Elena E Jungbauer Alois A Satzer Peter P
Biotechnology and bioengineering 20210325 9
Chemically defined media are reconstituted batchwise and stored in hold tanks until use. To avoid large hold tanks and batchwise production of media, we developed continuous on-demand reconstitutions directly from solids consisting of a hopper and a screw conveyor capable of feeding dry powdered media with the required precision ±5% at low dosing rates of 0.171 g min<sup>-1</sup> . A commercially available dry powdered cell culture medium was continuously fed over a duration of 12 h into a mixer ...[more]