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Protein Macrocyclization for Tertiary Structure Stabilization.

ABSTRACT: Proteins possess unique molecular recognition capabilities and enzymatic activities, features that are usually tied to a particular tertiary structure. To make use of proteins for biotechnological and biomedical purposes, it is often required to enforce their tertiary structure in order to ensure sufficient stability under the conditions inherent to the application of interest. The introduction of intramolecular crosslinks has proven efficient in stabilizing native protein folds. Herein, we give an overview of methods that allow the macrocyclization of expressed proteins, discussing involved reaction mechanisms and structural implications.

SUBMITTER: Haim A

PROVIDER: S-EPMC8453710 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Protein Macrocyclization for Tertiary Structure Stabilization.

Haim Anissa A Neubacher Saskia S Grossmann Tom N TN

Chembiochem : a European journal of chemical biology 20210621 17

Proteins possess unique molecular recognition capabilities and enzymatic activities, features that are usually tied to a particular tertiary structure. To make use of proteins for biotechnological and biomedical purposes, it is often required to enforce their tertiary structure in order to ensure sufficient stability under the conditions inherent to the application of interest. The introduction of intramolecular crosslinks has proven efficient in stabilizing native protein folds. Herein, we give ...[more]

PMID: 34060202

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