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Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning.


ABSTRACT: Here, we describe a protocol to identify escape mutants on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) receptor-binding domain (RBD) using a yeast screen combined with deep mutational scanning. Over 90% of all potential single S RBD escape mutants can be identified for monoclonal antibodies that directly compete with angiotensin-converting enzyme 2 for binding. Six to 10 antibodies can be assessed in parallel. This approach has been shown to determine escape mutants that are consistent with more laborious SARS-CoV-2 pseudoneutralization assays. For complete details on the use and execution of this protocol, please refer to Francino-Urdaniz et al. (2021).

SUBMITTER: Haas CM 

PROVIDER: S-EPMC8455247 | biostudies-literature |

REPOSITORIES: biostudies-literature

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