Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq.
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ABSTRACT:
SUBMITTER: Rodahl I
PROVIDER: S-EPMC8456112 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq.
Rødahl Inga I Gotley James J Andersen Stacey B SB Yu Meihua M Mehdi Ahmed M AM Christ Angelika N AN Hamilton-Williams Emma E EE Frazer Ian H IH Lukowski Samuel W SW Chandra Janin J
STAR protocols 20210917 4
Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021). ...[more]
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