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Conservation genomics reveals pesticide and pathogen exposure in the declining bumble bee Bombus terricola.

ABSTRACT: In recent years, many pollinators have experienced large population declines, which threaten food security and the stability of natural ecosystems. Bumble bees are particularly important because their ability to "buzz" pollinate and tolerate cooler conditions make them critical pollinators for certain plants and regions. Here, we apply a conservation genomics approach to study the vulnerable Bombus terricola. We sequenced RNA from 30 worker abdomens, 18 of which were collected from agricultural sites and 12 of which were collected from nonagricultural sites. We found transcriptional signatures associated with exposure to insecticides, with gene expression patterns suggesting that bumble bees were exposed to neonicotinoids and/or fipronil-two compounds known to negatively impact bees. We also found transcriptional signatures associated with pathogen infections. In addition to the transcriptomic analysis, we carried out a metatranscriptomic analysis and detected five pathogens in the abdomens of workers, three of which are common in managed honey bee and bumble bee colonies. Our conservation genomics study provides functional support for the role of pesticides and pathogen spillover in the decline of B. terricola. We demonstrate that conservation genomics is an invaluable tool which allows researchers to quantify the effects of multiple stressors that impact pollinator populations in the wild.

SUBMITTER: Tsvetkov N

PROVIDER: S-EPMC8457087 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:AimBumble bees (Hymenoptera: Apidae: Bombus) are economically and ecologically important pollinators in agroecosystems and wildland habitats. In the Nearctic region, there are approximately 41 species, of which the IUCN lists twelve species as vulnerable, endangered, or critically endangered. We conducted a standardized faunal survey to inform ongoing conservation efforts including petitions under review for the Endangered Species Act. Furthermore, we test the appropriateness of a methodology for accurately sampling bumble bee communities.LocationThe United States of America, including 31 sites in 15 states.MethodsWe surveyed 15 states in the summer of 2015 to assess community composition and relative species abundance at agricultural and seminatural sites throughout the United States. We collected approximately 100 bees, using aerial nets, from each of 31 sites and identified specimens to species, totaling 3,252 bees. We assessed our survey methodology to understand whether it accurately sampled the potential community of bumble bees at each site for utility in future monitoring efforts.ResultsAverage site species richness was 5.1 ± 2.05, and we detected 30 of the 41 species documented historically within the contiguous United States. Sampling a site beyond 100 bees rarely added additional species detections, whereas adding additional sampling sites within an ecoregion frequently increased the species richness for the ecoregion. Thirteen of the 30 species we detected each accounted for <1% of the total fauna, and two species accounted for 49.02% of all bees captured. Species richness and evenness increased with increasing latitude across communities.Main conclusionsSpecies diversity and evenness in bumble bees increases in northern latitudes and increasing elevation in the United States; however, a few common species tend to dominate communities while many species occur only in low numbers. The results of this survey effort can inform current conservation evaluations and planning.

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Conservation genomics reveals pesticide and pathogen exposure in the declining bumble bee Bombus terricola.

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