Identification of novel GPCR partners of the central melanocortin signaling.

ABSTRACT:

Objective

Homo- or heterodimerization of G protein-coupled receptors (GPCRs) generally alters the normal functioning of these receptors and mediates their responses to a variety of physiological stimuli in vivo. It is well known that melanocortin-3 receptor (MC3R) and melanocortin-4 receptor (MC4R) are key regulators of appetite and energy homeostasis in the central nervous system (CNS). However, the GPCR partners of MC3R and MC4R are not well understood. Our objective is to analyze single cell RNA-seq datasets of the hypothalamus to explore and identify novel GPCR partners of MC3R and MC4R and examine the pharmacological effect on the downstream signal transduction and membrane translocation of melanocortin receptors.

Methods

We conducted an integrative analysis of multiple

SUBMITTER: Li Y 

PROVIDER: S-EPMC8458986 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature