Genetic rearrangement during site specific integration event facilitates cell line development of a bispecific molecule.

ABSTRACT: Site specific integration (SSI) expression systems offer robust means of generating highly productive and stable cell lines for traditional monoclonal antibodies. As complex modalities such as antibody-like molecules comprised of greater than two peptides become more prevalent, greater emphasis needs to be placed on the ability to produce appreciable quantities of the correct product of interest (POI). The ability to screen several transcript stoichiometries could play a large role in ensuring high amounts of the correct POI. Here we illustrate implementation of an SSI expression system with a single site of integration for development and production of a multi-chain, bi-specific molecule. A SSI vector with a single copy of all of the genes of interest was initially selected for stable Chi

SUBMITTER: Tevelev B 

PROVIDER: S-EPMC8459265 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature