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LitR directly upregulates autoinducer synthesis and luminescence in Aliivibrio logei.


ABSTRACT: LitR is a master-regulator of transcription in the ainS/R and luxS/PQ quorum sensing (QS) systems of bacteria from Vibrio and Aliivibrio genera. Here, we for the first time directly investigated the influence of LitR on gene expression in the luxI/R QS system of psychrophilic bacteria Aliivibrio logei. Investigated promoters were fused with Photorhabdus luminescens luxCDABE reporter genes cassette in a heterological system of Escherichia coli cells, litR A. logei was introduced into the cells under control of P lac promoter. LitR has been shown to upregulate genes of autoinducer synthase (luxI), luciferase and reductase (luxCDABE), and this effect doesn't depend on presence of luxR gene. To a much lesser degree, LitR induces luxR1, but not the luxR2 - the main luxI/R regulator. Enhanced litR expression leads to an increase in a LuxI-autoinducer synthesis and a subsequent LuxR-mediated activation of the luxI/R QS system. Effect of LitR on luxI transcription depends on lux-box sequence in luxI promoter even in absence of luxR (lux-box is binding site of LuxR). The last finding indicates a direct interaction of LitR with the promoter in the lux-box region. Investigation of the effect of LitR A. logei on luxI/R QS systems of mesophilic Aliivibrio fischeri and psychrophilic Aliivibrio salmonicida showed direct luxR-independent upregulation of luxI and luxCDABE genes. To a lesser degree, it induces luxR A. fischeri and luxR1 A. salmonicida. Therefore, we assume that the main role of LitR in cross-interaction of these three QS systems is stimulating the expression of luxI.

SUBMITTER: Bazhenov S 

PROVIDER: S-EPMC8462370 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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LitR directly upregulates autoinducer synthesis and luminescence in <i>Aliivibrio logei</i>.

Bazhenov Sergey S   Melkina Olga O   Fomin Vadim V   Scheglova Ekaterina E   Krasnik Pavel P   Khrulnova Svetlana S   Zavilgelsky Gennadii G   Manukhov Ilya I  

PeerJ 20210921


LitR is a master-regulator of transcription in the <i>ainS/R</i> and <i>luxS/PQ</i> quorum sensing (QS) systems of bacteria from <i>Vibrio</i> and <i>Aliivibrio</i> genera. Here, we for the first time directly investigated the influence of LitR on gene expression in the <i>luxI/R</i> QS system of psychrophilic bacteria <i>Aliivibrio logei</i>. Investigated promoters were fused with <i>Photorhabdus luminescens luxCDABE</i> reporter genes cassette in a heterological system of <i>Escherichia coli</  ...[more]

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