Dataset Information

Isolation, identification antimicrobial susceptibility and associated risk factors of Salmonella in semi-intensive poultry farms of Kafa zone, Southwest Ethiopia.

ABSTRACT: Salmonellosis is one of the major causes of poultry disease. The study aimed to isolate, identify, determine susceptibility and associated risk factors of salmonella specious in semi-intensive poultry farms of Kafa zone, southwest Ethiopia. A cross-sectional study was conducted on four purposively selected districts. Three farms were randomly selected per district and fecal samples were taken from a total of 302 chickens. Questionnaire was administered to farm owners and data was analyzed using STATA statistical software package. The overall prevalence of Salmonella enterica species in Kafa zone was 9.27% with Gimbo district 10.39%, Bita district 10.66%, Shishoende district 12% and Chena district 4%. Source of chickens, farm types and breed risk factors showed significant association (P < 0.05) with the disease prevalence. Having diarrhea and continuous farm systems significantly associated (P < 0.05). All isolates were 100% resistant to Oxtytetracycline and Ampicillin. Among 28 isolated Salmonella enterica species, 92.85% (n = 26) of them were showed multidrug resistance while 2 (7.14%) of them showed extensively drug resistance. Half of multidrug-resistant isolates were resistant to 5-6 antimicrobials, while 7.14% of isolates showed resistance to 7 antimicrobials. This study shows prevalence of Salmonella and its association with the breed, farm type, source of chicken and presence of diarrhea. A high antimicrobial resistance observed shows presence of concerns due to the emergence of Antimicrobial Resistance (AMR) in the poultry farms. Therefore, awareness should be created to the farmers on measures to avoid the risk factors of poultry disease and the occurrence of antimicrobials resistance in poultry farms.

SUBMITTER: Abda S

PROVIDER: S-EPMC8473537 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Salmonella is one of the most important foodborne zoonotic pathogens, and becoming multidrug-resistant (MDR), which represents a serious public health concern worldwide. This study aimed to identify the circulating MDR strains of Salmonella through cutting edge molecular techniques including gene specific PCR, RAPD-PCR, ribosomal gene sequencing, and multilocus sequence types (MLST) in the poultry industry of Bangladesh. Two hundred Salmonella isolates were retrieved from 154 samples comprising droppings (n = 60), cloacal swabs (n = 60), feeds (n = 14), feeding water (n = 14), and handler's swab (n = 6) from 14 commercial layer farms of Bangladesh. The isolates were confirmed as Salmonella through invA gene specific PCR, and further genotyping was done by RAPD-PCR, and 16S rRNA sequencing. The isolates were distributed into 18 different genotypes according to RAPD typing. The phylogenetic analysis identified three diverging phylogroups such as S. enterica Litchfield, S. enterica Enteritidis and S. enterica Kentucky with 11, 8, and 6 strains, respectively. The in vitro antibiogram profiling the Salmonella isolates through disc diffusion method using 13 commercially available antibiotics revealed highest resistance against doxycycline (91.5%) followed by tetracycline and ampicillin (86.0%, in each), and 72.0% isolates as MDR, being resistant to ≥ 5 antibiotics. The MLST typing was carried out based on the PCR amplification of seven housekeeping genes (aroC, hisD, hemD, purE, secA, thrA, and dnaN). MLST typing also revealed three sequence types (STs) such as ST11, ST198, and ST214 in these isolates, and eBURST analysis showed ST11 as the founder genotype. The three STs were highly resistant to tetracyclines and quinolone group of antibiotics, and all of the isolates harboring S. enterica Litchfield showed the highest resistance. Circulating common MLSTs with MDR properties in different farms confirmed the possibility of a common route of intra-farm transmission. We report for the first time of the association serovar Litchfield (ST11) in avian salmonellosis with MDR properties which is an urgent public health concern in Bangladesh.

Project description:Background Indiscriminate use of antimicrobials for the prevention and treatment of bacterial infection in animals is a common practice in Nigeria as in other developing countries. These antimicrobials are purchased over the counter without restrictions and often administered in form of medicated feedstuffs. In Nigeria, like most developing countries, antimicrobial prescription data are not routinely collected or reported at the farm level, instead import data are used in reporting antimicrobial consumption. Farmers can be useful sources of data on the use of antimicrobial agents by class, animal species, production type and age. The objective of the study was to determine the knowledge, attitude and practices of poultry farmers on antimicrobial resistance and to generate data on antimicrobial use (AMU) in poultry farms in Plateau and Oyo states in accordance with the guidelines of the World Organization for Animal Health (WOAH). Methods A questionnaire used by the Food and Agriculture Organization (FAO) of the United Nations in Ghana was adopted and modified to collect data on the knowledge, attitude and practices of farmers on AMR and AMU and to collect AMU data from selected poultry farms. A focus group discussion (FGD) was conducted in Plateau state with poultry farmers and representatives from the state veterinary services, using a checklist. The aim of the FGD was to have an idea on antimicrobial use among poultry farmers and to generate additional questions that might be added to the questionnaire. Stratified random sampling technique was used to select 50 farms from Plateau and Oyo states, using the list of registered poultry farms in the two states as sampling frame. Results Ninety eight percent (98%) of farmers gave antibiotics as prophylactic treatment to day old chicks. There were 47 different products used in the two states within the study period. We observed that five classes of antibiotics (Tetracyclines, Penicillins, Aminoglycosides, Polypeptides and Fluoroquinolone) were used in the two states. A total of 351 kg of active ingredients from seven different classes, namely: tetracyclines, penicillins, aminoglycosides, polypeptide, fluoroquinolones, amphenicol and macrolides were recorded from the two states. Some products contained cocktail of antibiotics, having up to six different classes with very high concentration of active ingredients which are not in the list of registered antimicrobials reported to WOAH. Conclusion The concept used for this survey proved that the approach can be applied for AMU surveillance in the animal health sector. It also provided insight on farmers’ knowledge and practices with regards to the use of antimicrobials which is missing in the national import data. The need for “stronger” antibiotics was identified as one of the drivers of antibiotic resistance. Supplementary Information The online version contains supplementary material available at 10.1186/s13756-023-01235-x.

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Isolation, identification antimicrobial susceptibility and associated risk factors of Salmonella in semi-intensive poultry farms of Kafa zone, Southwest Ethiopia.

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