Ontology highlight
ABSTRACT:
SUBMITTER: Barnes LF
PROVIDER: S-EPMC8476707 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Barnes Lauren F LF Draper Benjamin E BE Chen Yu-Ting YT Powers Thomas W TW Jarrold Martin F MF
Molecular therapy. Methods & clinical development 20210826
Recombinant adeno-associated virus (rAAV) has emerged as an important gene therapy vector with many clinical trials currently in progress. Analytical characterization and quantitation of particle content remain challenges in both the development and production of rAAV vectors. In this study, charge detection mass spectrometry (CDMS) and gel electrophoresis are used to characterize the DNA content of recombinant AAV8 (rAAV8) vectors with a wide range of target genome sizes. We show that the diffe ...[more]