Unknown

Dataset Information

0

New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses.


ABSTRACT: A new chloride-sensitive red fluorescent protein derived from Entacmaea quadricolor is described. We found that mBeRFP exhibited moderate sensitivity to chloride and, via site-directed mutagenesis (S94V and R205Y), we increased the chloride affinity by more than an order of magnitude (kd = 106 ± 6 mM) at physiological pH. In addition, cis-trans isomerization of the chromophore produces a dual emission band with different chloride sensitivities, which allowed us to develop a ratiometric methodology to measure intracellular chloride concentrations.

SUBMITTER: Salto R 

PROVIDER: S-EPMC8478333 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC5950754 | biostudies-literature
| S-EPMC2818747 | biostudies-literature
| S-EPMC2855763 | biostudies-literature
| S-EPMC4226733 | biostudies-literature
| S-EPMC1995760 | biostudies-literature
| S-EPMC6366848 | biostudies-literature
| S-EPMC6334773 | biostudies-literature
| S-EPMC6649077 | biostudies-literature
| S-EPMC7930027 | biostudies-literature
| S-EPMC122988 | biostudies-literature