Increased Monocyte-Derived CD11b⁺ Macrophage Subpopulations Following Cigarette Smoke Exposure Are Associated With Impaired Bleomycin-Induced Tissue Remodelling.

ABSTRACT:

Rationale

The accumulation of macrophages in the airways and the pulmonary interstitium is a hallmark of cigarette smoke-associated inflammation. Notably, pulmonary macrophages are not a homogenous population but consist of several subpopulations. To date, the manner in which cigarette smoke exposure affects the relative composition and functional capacity of macrophage subpopulations has not been elucidated.

Methods

Using a whole-body cigarette smoke exposure system, we investigated the impact of cigarette smoke on macrophage subpopulations in C57BL/6 mice using flow cytometry-based approaches. Moreover, we used bromodeoxyuridine labelling plus Il1a^-/- and Il1r1^-/- mice to assess the relative contribution of local proliferation and monocy

SUBMITTER: Cass SP 

PROVIDER: S-EPMC8481926 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature