Chemically Crosslinked Methylcellulose Substrates for Cell Sheet Engineering.

ABSTRACT: Methylcellulose (MC) hydrogels have been successfully proposed in the field of cell sheet engineering (CSE), allowing cell detachment from their surface by lowering the temperature below their transition temperature (T_t). Among the main limitations of pristine MC hydrogels, low physical stability and mechanical performances limit the breadth of their potential applications. In this study, a crosslinking strategy based on citric acid (CA) was used to prepare thermoresponsive MC hydrogels, with different degrees of crosslinking, to exploit their possible use as substrates in CSE. The investigated amounts of CA did not cause any cytotoxic effect while improving the mechanical performance of the hydrogels (+11-fold increase in E, compared to control MC). The possibility to obtain ce

SUBMITTER: Bonetti L 

PROVIDER: S-EPMC8482237 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature