Unknown

Dataset Information

0

Functional Th1-oriented T follicular helper cells that infiltrate human breast cancer promote effective adaptive immunity.


ABSTRACT: We previously demonstrated that tumor-infiltrating lymphocytes (TIL) in human breast cancer sometimes form organized tertiary lymphoid structures (TLS) characterized by CXCL13-producing T follicular helper (Tfh) cells. The present study found that CD4+ Tfh TIL, CD8+ TIL, and TIL-B, colocalizing in TLS, all express the CXCL13 receptor CXCR5. An ex vivo functional assay determined that only activated, functional Th1-oriented Tfh TIL (PD-1hiICOSint phenotype) provide help for immunoglobulin and IFN-γ production. A functional Tfh TIL presence signals an active TLS, characterized by humoral (immunoglobulins, Ki-67+ TIL-B in active germinal centers) and cytotoxic (GZMB+CD8+ and GZMB+CD68+ TIL plus Th1 gene expression) immune responses. Analysis of active versus inactive TLS in untreated patients revealed that the former are associated with positive clinical outcomes. TLS also contain functional T follicular regulatory (Tfr) TIL, which are characterized by a CD25+CXCR5+GARP+FOXP3+ phenotype and a demethylated FOXP3 gene. Functional Tfr inhibited functional Tfh activities via a glycoprotein A repetitions predominant (GARP)-associated TGF-β-dependent mechanism. The activity of tumor-associated TLS was dictated by the relative balance between functional Tfh TIL and functional Tfr TIL. These data provide mechanistic insight into TLS processes orchestrated by functional Th1-oriented Tfh TIL, including TIL-B and CD8+ TIL activation and immunological memory generation. Tfh TIL, regulated by functional Tfr TIL, are an expected key target of PD-1/PD-L1 blockade.

SUBMITTER: Noel G 

PROVIDER: S-EPMC8483751 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6385600 | biostudies-literature
| S-EPMC4023883 | biostudies-other
| S-EPMC8484368 | biostudies-literature
| S-EPMC6700230 | biostudies-literature
| S-EPMC10869348 | biostudies-literature
| S-EPMC3601086 | biostudies-literature
| S-EPMC3960397 | biostudies-literature
| S-EPMC3696556 | biostudies-literature
| S-EPMC4758890 | biostudies-literature
| S-EPMC8206650 | biostudies-literature