Project description:Whole blood was collected from cynomolgus macaques into PAXgene Blood RNA tubes (PreAnalytiX) and stored at –20 °C until RNA extraction. Animals were divided into three vaccine groups: mRNA influenza vaccine (n = 5), Vaxigrip (n = 4), and Fluad (n = 5). Samples were taken at baseline (0 h), 24 h post-vaccination, and for some animals at 24 h after a booster, resulting in 35 samples in total. RNA was extracted using Trizol reagent (Ambion) with Tissuelyser (QIAGEN) disruption, followed by labeling with the Quick Amp Labeling Kit (Agilent) and purification with RNeasy spin columns (QIAGEN). Labeled cRNA was hybridized to Agilent Rhesus Macaque Gene Expression Microarrays v2 (G2519F-026806), scanned on an Agilent DNA Microarray Scanner (G2505C), and processed with Agilent Feature Extraction Software.
Project description:The goal of this study was to examine differential gene expression in human platelets, focusing on it's relationship to flu vaccine response and how it may vary with age.