Comparison of polynomial fitting versus single time point analysis of ECIS data for barrier assessment.

ABSTRACT: Electrical cell-substrate impedance sensing (ECIS) is an in vitro methodology for measuring the barrier integrity of a variety of cell types, including pulmonary endothelial cells. These experiments are frequently used for in vitro assessment of lung injury. The data derived from ECIS experiments consists of repeated measures of resistance across an endothelial monolayer. As such, these data reflect the dynamic changes in electrical resistance that occur over time. Currently methodologies for assessing ECIS data rely on single point assessments of barrier function, such as the maximal drop in trans-endothelial electrical resistance (TER_Max ). However, this approach ignores the myriad of changes in resistance that occur before and after the TER_Max data point. Herein, w

SUBMITTER: Suresh K 

PROVIDER: S-EPMC8488550 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature