Identification of disease- and headache-specific mediators and pathways in migraine using blood transcriptomic and metabolomic analysis.

ABSTRACT:

Background

Recent data suggest that gene expression profiles of peripheral white blood cells can reflect changes in the brain. We aimed to analyze the transcriptome of peripheral blood mononuclear cells (PBMC) and changes of plasma metabolite levels of migraineurs in a self-controlled manner during and between attacks.

Methods

Twenty-four patients with migraine were recruited and blood samples were collected in a headache-free (interictal) period and during headache (ictal) to investigate disease- and headache-specific alterations. Control samples were collected from 13 age- and sex-matched healthy volunteers. RNA was isolated from PBMCs and single-end 75 bp RNA sequencing was performed using Illumina NextSeq 550 instrument followed by gene-level differential expression anal

SUBMITTER: Aczel T 

PROVIDER: S-EPMC8493693 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature