Direct IgG epitope mapping on bacterial AB toxins by cryo-EM.
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ABSTRACT:
SUBMITTER: Nguyen T
PROVIDER: S-EPMC8496303 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Direct IgG epitope mapping on bacterial AB toxins by cryo-EM.
STAR protocols 20211001 4
This cryo-EM protocol was used to determine the B cell epitope map on the CdtB subunit of typhoid toxin, an A<sub>2</sub>B<sub>5</sub> toxin secreted by <i>Salmonella</i> Typhi during infection. Immunoglobulin G (IgG) was directly mixed with typhoid toxin in this protocol, different from our previous cryo-EM protocol that uses the Fab fragments in place of IgG. This simple approach requires smaller amounts of materials, supporting the broader use of this protocol for determining antibody recogni ...[more]
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