Dataset Information

Influence of the Environment on the Distribution and Quality of Gentiana dahurica Fisch.

ABSTRACT: Gentiana dahurica Fisch. is a characteristic medicinal plant found in Inner Mongolia, China. To meet the increase in market demand and promote the development of medicinal plant science, we explored the influence of the environment on its distribution and the quantity of its active compounds (loganic acid and 6'-O-β-D-glucosylgentiopicroside) to find suitable cultivation areas for G. dahurica. Based on the geographical distribution of G. dahurica in Inner Mongolia and the ecological factors that affect its growth, identified from the literature and field visits, a boosted regression tree (BRT) was used to model ecologically suitable areas in the region. The relationship between the content of each of active compound in the plant and ecological factors was also established for Inner Mongolia using linear regression. The results showed that elevation and soil type had the most significant influence on the distribution of G. dahurica-their relative contribution was 30.188% and 28.947%, respectively. The factors that had the greatest impact on the distribution of high-quality G. dahurica were annual precipitation, annual mean temperature, and temperature seasonality. The results of BRT and linear regression modeling showed that suitable areas for high-quality G. dahurica included eastern Ordos, southern Baotou, Hohhot, southern Wulanchabu, southern Xilin Gol, and central Chifeng. However, there were no significant correlations between the contents of loganic acid and 6'-O-β-D-glucosylgentiopicroside and the ecological factors. This study explored the influence of the environment on the growth and quantity of active compounds in G. dahurica to provide guidance for coordinating the development of medicinal plant science.

SUBMITTER: Zhang M

PROVIDER: S-EPMC8503573 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Glycyrrhiza uralensis Fisch. is known as a common Chinese medicinal herb used to harmonize the effects of other ingredients in most Chinese herbal prescriptions. The rapid production of flavonoids in vitro remains unknown in G. uralensis Fisch. To investigate the in vitro adventitious root regeneration and flavonoid accumulation characteristics in G. uralensis for restrictions on collecting wild plants, suspension cultural and freezing microtomy with histochemical assays were carried out. We reported that multiple adventitious roots were initiated from hypocotyls and stems of G. uralensis. Indole-3-butyric acid (IBA) was more conducive than NAA (1-naphthaleneacetic acid) in inducing G. uralensis adventitious roots, but the addition of 6-BA (6-benzylaminopurine) and KT (kinetin) suppressed the formation of adventitious roots. While the concentration of IBA was 1.0 mg L-1, the flavonoid content and yield were the highest at 19.96 mg g-1 and 1.23 mg g-1, respectively. The optimum medium for adventitious root induction was 1/4-strength Murashige and Skoog's medium containing 0.1 mg L-1 IBA. The content of flavonoids in adventitious roots and apicals cultured in vitro was higher than that in suspension callus, reaching 3.87 times the callus flavonoid content. The histochemical localization of flavonoids showed that G. uralensis flavonoids mainly distributed in the epidermal parenchyma cells of the callus outer layers and gradually accumulated in cell wall and cell gaps of the epidermis and endodermis of adventitious roots along with the primary growth of adventitious roots, indicating that there were no flavonoids in the roots at the early stage of adventitious roots formation. The results showed that calli inducing adventitious roots and apicals for 30 days obtained the highest yield of flavonoid, indicating effective production for flavonoids instead of wild culture. AlCl3 ethanol solution was better than NaOH aqueous solution in terms of chromogenic and localization effects. We concluded that the highest yield of flavonoid and effective production for flavonoid instead of wild culture could be obtained from calli inducing adventitious roots and apicals.

Dataset Information

Influence of the Environment on the Distribution and Quality of Gentiana dahurica Fisch.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets