Multiple Variant Calling Pipelines in Wheat Whole Exome Sequencing.

ABSTRACT: The highly challenging hexaploid wheat (Triticum aestivum) genome is becoming ever more accessible due to the continued development of multiple reference genomes, a factor which aids in the plight to better understand variation in important traits. Although the process of variant calling is relatively straightforward, selection of the best combination of the computational tools for read alignment and variant calling stages of the analysis and efficient filtering of the false variant calls are not always easy tasks. Previous studies have analyzed the impact of methods on the quality metrics in diploid organisms. Given that variant identification in wheat largely relies on accurate mining of exome data, there is a critical need to better understand how different methods affect the analysis o

SUBMITTER: Cagirici HB 

PROVIDER: S-EPMC8509018 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature