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Mitochondrial Genomes, Phylogenetic Associations, and SNP Recovery for the Key Invasive Ponto-Caspian Amphipods in Europe.

ABSTRACT: The Ponto-Caspian region is the main donor of invasive amphipods to freshwater ecosystems, with at least 13 species successfully established in European inland waters. Dikerogammarus spp. and Pontogammarus robustoides are among the most successful, due to their strong invasive impact on local biota. However, genomic knowledge about these invaders is scarce, while phylogeography and population genetics have been based on short fragments of mitochondrial markers or nuclear microsatellites. In this study, we provide: (i) a reconstruction of six mitogenomes for four invasive gammarids (D. villosus, D. haemobaphes, D. bispinosus, and P. robustoides); (ii) a comparison between the structure of the newly obtained mitogenomes and those from the literature; (iii) SNP calling rates for individual D. villosus and D. haemobaphes from different invasion sites across Europe; and (iv) the first time-calibrated full mitogenome phylogeny reconstruction of several Ponto-Caspian taxa. We found that, in comparison to other gammarids, the mitogenomes of Ponto-Caspian species show a translocation between the tRNA-E and tRNA-R positions. Phylogenetic reconstruction using the mitogenomes identified that Ponto-Caspian gammarids form a well-supported group that originated in the Miocene. Our study supports paraphyly in the family Gammaridae. These provided mitogenomes will serve as vital genetic resources for the development of new markers for PCR-based identification methods and demographic studies.

SUBMITTER: Mamos T

PROVIDER: S-EPMC8509019 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:The degree of dietary specialization has a fundamental impact on the ecological function and interactions of suspension feeders. While niche differentiation by food particle size is common among obligate suspension feeders, its role is not evident in facultative ones. In this study, we aimed at providing new insights on the matter by focusing on sympatric mysid species. As mysids use different mechanisms for raptorial and filter-feeding, they represent a more adequate model system than for example, the more extensively studied copepods. We made morphological measurements on the 4 coexisting invasive Ponto-Caspian mysid species to determine the areas and mesh sizes of their filters. We also quantified their clearance rates on the microalga Cryptomonas sp. in a laboratory experiment to reveal how morphological differences manifest in their overall filtering capacity. We found relatively small but consistent differences in the primary filter area among the species, indicating that morphological constraints due to the enclosed position of the setae might limit the possibility for differentiation. The primary filter mesh sizes were small in all 4 species (0.69-2.73 μm) with moderate but consistent intraspecific differences, suggesting that the benefit of being able to capture small particles might outweigh the pressure for differentiation. The observed clearance rates were in accordance with the morphological characteristics of the species, highlighting that auxiliary filters (present in one of the species, Limnomysis benedeni) are needed to increase filtering capacity considerably. Our study confirmed that food particle size can contribute to the niche differentiation of facultative filter feeders, but also indicated that they can tolerate a higher overlap than obligate ones. The observed differences were related to the habitat preferences and predatory potentials of the species, suggesting that complementarity among the different niche axes might further facilitate their coexistence.

Project description:Temperature is a crucial factor determining biology and ecology of poikilothermic animals. It often constitutes an important barrier for invasive species originating from different climate zones but, on the other hand, may facilitate the invasion process of animals with wide thermal preferences and high resistance to extreme temperatures. In our experimental study, we investigated the thermal behaviour of two Ponto-Caspian amphipod crustaceans-Dikerogammarus villosus and Dikerogammarus haemobaphes. Both species are known to live under a wide range of thermal conditions which may promote their invasion. Moreover, both these amphipods are hosts for microsporidian parasites which co-evolved with them within the Ponto-Caspian region and spread in European waters. As the presence of a parasite may influence the thermal preferences of its host, we expected to observe behavioural changes in infected individuals of the studied amphipods leading to (1) behavioural fever (selecting a warmer habitat) or (2) anapyrexia (selecting a colder habitat). The experiment (N = 20) was carried out for 30 min in a 100 cm. 20 cm from boths sides were not avaliable for amphipods long thermal gradient (0-40 °C), using 30 randomly selected adult amphipod individuals of one species. At the end of each trial, we checked the position of amphipods along the gradient and determined their sex and infection status (uninfected or infected by one of microsporidium species). D. villosus was infected with Cucumispora dikerogammari whereas D. haemobaphes was a host for C. dikerogammari, Dictyocoela muelleri or D. berillonum. Thermal preferences of amphipods depended on their species and sex. Females of D. villosus preferred warmer microhabitats (often much above 30 °C) than conspecific males and females of D. haemobaphes, whereas no significant differences were found among males of both species and both sexes of D. haemobaphes. Moreover, infected males of D. villosus stayed in warmer water more often than uninfected males of this species, selecting temperatures higher than 30 °C, which may be explained either as a behavioural fever constituting a defence mechanism of a host against the infection, or as a parasite manipulation of the host behaviour increasing the parasite fitness. On the other hand, none of the parasite species affected the thermal preferences of D. haemobaphes, including also C. dikerogammari, changing the behaviour of D. villosus. Our research presents the complexity of the thermal behaviour of studied amphipods and the evidence that microsporidia may trigger a change in temperature preferendum of their host species and those observations may be the result of different host-parasite coevolution time which may vary for the two host species (Poulin, 2010).

Project description:Suspension feeders play pivotal roles in the nutrient cycling of almost all aquatic ecosystems. Since sufficiently large differences in the filter mesh size (FMS) can lead to different food web positions, the inter- and intraspecific variability of this trait might be of community-level importance. The aim of this study was to quantify the range of FMS variation within the three invasive Ponto-Caspian Chelicorophium species based on a large material representing various conditions (1,224 specimens from 40 samples across Central Europe), characterize the components of variation within populations, identify the main factors determining intraspecific differences, and reveal how intraspecific variation affects the FMS overlaps among species. The FMS of the most widespread invader, C. curvispinum, varied within the broadest range (between 2.34-8.28 μm, compared to 2.51-5.97 μm in C. robustum and 1.08-3.23 μm in C. sowinskyi); nevertheless, the contribution of intraspecific plasticity to the invasion success of the species is not evident based on the present study. The within-individual variability of FMS increased with the individual mean of the trait and decreased with body size; however, it showed little differences among samples. The among-individual variation within samples could be partitioned into components related to body size (ontogenetic niche shift/differences among cohorts) and sex (ecological sexual dimorphism) as well as a seemingly random component (individual specialization), varying widely in extent and relative contributions. The FMS of C. curvispinum was significantly larger in the presence of C. sowinskyi than in allopatry, likely reflecting character displacement; however, it did not show further increase when C. robustum was also present. Similar differences could not be observed in C. sowinskyi. The FMS ranges of C. curvispinum and C. robustum never overlapped with that of C. sowinskyi in co-occurrence despite the considerable intraspecific differences among sites, suggesting that their interaction can be seen as a clear case of niche differentiation by food particle size. On the contrary, the strong overlaps observed between C. curvispinum and C. robustum indicate that other factors might play the primary role in their coexistence. The studied species appear to be suitable model organisms for identifying the drivers and mechanisms of FMS variability.

Project description:Predators shape prey populations by elimination of individuals (consumptive effects) and by inducing modifications in prey behaviour, physiology or morphology (NCE-non-consumptive effects). Due to the resource allocation to defence, decreased feeding and higher stress, the costs of predator NCEs can be considerable. Therefore, the resistance to NCEs may be crucial for population growth and interspecific competition. We tested the resistance of Ponto-Caspian gammarids Dikerogammarus villosus and Pontogammarus robustoides to NCEs imposed by their predator, the racer goby Babka gymnotrachelus. As D. villosus is often avoided by predators in the presence of alternative food, we hypothesised that it would bear lower behavioural and physiological costs of anti-predator responses. We tested gammarid feeding in short-time experiments (2-4 h) with food (chironomid larvae) located at various distances from the stony shelter (to enforce food searching, Experiment I) or in the direct gammarid proximity (no searching needed, Experiment II). Moreover, we checked the predator effect on gammarid growth in a 2-week Experiment III. Both gammarids exposed to predators reduced feeding efficiency outside the shelter (Experiment I). Contrary to our expectations, the response of D. villosus was stronger. When food was provided in their direct proximity (Experiment II), the feeding of both species was unaffected by predators, indicating that a shelter supplied with food can reduce predator NCEs. The growth of P. robustoides was reduced in the presence of predators (Experiment III), whereas that of D. villosus was unaffected. Although D. villosus has a more effective defence strategy than P. robustoides, it bears similar or even higher behavioural costs of NCEs. However, it exhibits the higher resistance to the long-term predator presence, sustaining its growth rate under such conditions. This may be one of the factors contributing to the great invasion success of D. villosus, currently taking place in European fresh waters.

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Mitochondrial Genomes, Phylogenetic Associations, and SNP Recovery for the Key Invasive Ponto-Caspian Amphipods in Europe.

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