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Ions in the Deep Subsurface of Earth, Mars, and Icy Moons: Their Effects in Combination with Temperature and Pressure on tRNA-Ligand Binding.

ABSTRACT: The interactions of ligands with nucleic acids are central to numerous reactions in the biological cell. How such reactions are affected by harsh environmental conditions such as low temperatures, high pressures, and high concentrations of destructive ions is still largely unknown. To elucidate the ions' role in shaping habitability in extraterrestrial environments and the deep subsurface of Earth with respect to fundamental biochemical processes, we investigated the effect of selected salts (MgCl₂, MgSO₄, and Mg(ClO₄)₂) and high hydrostatic pressure (relevant for the subsurface of that planet) on the complex formation between tRNA and the ligand ThT. The results show that Mg²⁺ salts reduce the binding tendency of ThT to tRNA. This effect is largely due to the interaction of ThT with the salt anions, which leads to a strong decrease in the activity of the ligand. However, at mM concentrations, binding is still favored. The ions alter the thermodynamics of binding, rendering complex formation that is more entropy driven. Remarkably, the pressure favors ligand binding regardless of the type of salt. Although the binding constant is reduced, the harsh conditions in the subsurface of Earth, Mars, and icy moons do not necessarily preclude nucleic acid-ligand interactions of the type studied here.

SUBMITTER: Jahmidi-Azizi N

PROVIDER: S-EPMC8509373 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:The emerging field of astropedology is the study of ancient soils on Earth and other planetary bodies. Examination of the complex factors that control the preservation of organic matter and other biosignatures in ancient soils is a high priority for current and future missions to Mars. Though previously defined by biological activity, an updated definition of soil as planetary surfaces altered in place by biological, chemical or physical processes was adopted in 2017 by the Soil Science Society of America in response to mounting evidence of pedogenic-like features on Mars. Ancient (4.1-3.7 billion year old [Byr]) phyllosilicate-rich surface environments on Mars show evidence of sustained subaerial weathering of sediments with liquid water at circumneutral pH, which is a soil-forming process. The accumulation of buried, fossilized soils, or paleosols, has been widely observed on Earth, and recent investigations suggest paleosol-like features may be widespread across the surface of Mars. However, the complex array of preservation and degradation factors controlling the fate of biosignatures in paleosols remains unexplored. This paper identifies the dominant factors contributing to the preservation and degradation of organic carbon in paleosols through the geological record on Earth, and offers suggestions for prioritizing locations for in situ biosignature detection and Mars Sample Return across a diverse array of potential paleosols and paleoenvironments of early Mars. A compilation of previously published data and original research spanning a diverse suite of paleosols from the Pleistocene (1 Myr) to the Archean (3.7 Byr) show that redox state is the predominant control for the organic matter content of paleosols. Most notably, the chemically reduced surface horizons (layers) of Archean (2.3 Byr) paleosols have organic matter concentrations ranging from 0.014-0.25%. However, clay mineralogy, amorphous phase abundance, diagenetic alteration and sulfur content are all significant factors that influence the preservation of organic carbon. The surface layers of paleosols that formed under chemically reducing conditions with high amounts of iron/magnesium smectites and amorphous colloids should be considered high priority locations for biosignature investigation within subaerial paleoenvironments on Mars.

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Ions in the Deep Subsurface of Earth, Mars, and Icy Moons: Their Effects in Combination with Temperature and Pressure on tRNA-Ligand Binding.

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