Structure-based charge calculations for predicting isoelectric point, viscosity, clearance, and profiling antibody therapeutics.
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ABSTRACT:
SUBMITTER: Thorsteinson N
PROVIDER: S-EPMC8510563 | biostudies-literature | 2021 Jan-Dec
REPOSITORIES: biostudies-literature
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Structure-based charge calculations for predicting isoelectric point, viscosity, clearance, and profiling antibody therapeutics.
Thorsteinson Nels N Gunn John R JR Kelly Kenneth K Long Will W Labute Paul P
mAbs 20210101 1
The effect of hydrophobicity on antibody aggregation is well understood, and it has been shown that charge calculations can be useful for high-concentration viscosity and pharmacokinetic (PK) clearance predictions. In this work, structure-based charge descriptors are evaluated for their predictive performance on recently published antibody pI, viscosity, and clearance data. From this, we devised four rules for therapeutic antibody profiling which address developability issues arising from hydrop ...[more]
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