Beta-Lactam Antibiotic Discrimination Using a Macromolecular Sensor in Water at Neutral pH.

ABSTRACT: Penicillins and cephalosporins belong to the β-lactam antibiotic family, which accounts for more than half of the world market for antibiotics. Misuse of antibiotics harms human health and the environment. Here, we describe an easy, fast, and sensitive optical method for the sensing and discrimination of two penicillin and five cephalosporin antibiotics in buffered water at pH 7.4, using fifth-generation poly (amidoamine) (PAMAM) dendrimers and calcein, a commercially available macromolecular polyelectrolyte and a fluorescent dye, respectively. In aqueous solution at pH 7.4, the dendrimer and dye self-assemble to form a sensor that interacts with carboxylate-containing antibiotics through electrostatic interaction, monitored through changes in the dye's spectroscopic properties. This respo

SUBMITTER: Xu Y 

PROVIDER: S-EPMC8512602 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature