Dataset Information

EF24 exerts cytotoxicity against NSCLC via inducing ROS accumulation.

ABSTRACT:

Background

The role of Diphenyldifluoroketone (EF24), a synthetic analogue of curcumin with noteworthy antitumor potential, remains unclear in non-small cell lung cancer (NSCLC). Herein, the inhibitory effect of EF24 on NSCLC and its mechanism were studied.

Methods

Cytotoxicity was measured by MTT assay, colony formation assay and xenograft model. Cell apoptosis and reactive oxygen species (ROS) level were quantified by flow cytometer. Protein level was detected by western blot assay. Mitochondria and autophagosomes were observed using transmission electron microscope and confocal microscopy.

Results

In-vitro, EF24 significantly induced proliferation inhibition, apoptosis, mitochondrial fission and autophagy of NSCLC cell lines. These cytotoxic effects were significantly attenuated by two reactive oxygen species (ROS) scavengers, indicating its anti-cancer effects largely depend on ROS accumulation. In-vivo, EF24 inhibited tumor growth in a dose-dependent manner. Moreover, no pathological changes of heart, lung, spleen, kidney and liver of mice were observed. Collectively, EF24 induced ROS accumulation, in turn activates cell apoptosis, and then exerts its cytotoxicity on NSCLC cells.

Conclusions

The results showed that EF24 exerted cytotoxicity against NSCLC via ROS accumulation. Thus, EF24 might serve as a potential anti-cancer agent for the treatment of NSCLC.

SUBMITTER: Chang M

PROVIDER: S-EPMC8513219 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Publications

EF24 exerts cytotoxicity against NSCLC via inducing ROS accumulation.

Chang Minghui M Shang Ming M Yuan Fang F Guo Wei W Wang Cuijuan C

Cancer cell international 20211012 1

<h4>Background</h4>The role of Diphenyldifluoroketone (EF24), a synthetic analogue of curcumin with noteworthy antitumor potential, remains unclear in non-small cell lung cancer (NSCLC). Herein, the inhibitory effect of EF24 on NSCLC and its mechanism were studied.<h4>Methods</h4>Cytotoxicity was measured by MTT assay, colony formation assay and xenograft model. Cell apoptosis and reactive oxygen species (ROS) level were quantified by flow cytometer. Protein level was detected by western blot as ...[more]

PMID: 34641863

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Dataset Information

EF24 exerts cytotoxicity against NSCLC via inducing ROS accumulation.

Background

Methods

Results

Conclusions

Publications

EF24 exerts cytotoxicity against NSCLC via inducing ROS accumulation.

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