Sex-Differences in the Oxygenation Levels of Intercostal and Vastus Lateralis Muscles During Incremental Exercise
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ABSTRACT:
SUBMITTER: Espinosa-Ramirez M
PROVIDER: S-EPMC8517227 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:BACKGROUND: The trapezius muscle is a neck muscle that is susceptible to chronic pain conditions associated with repetitive tasks, commonly referred to as chronic work-related myalgia, hence making the trapezius a muscle of clinical interest. To provide a basis for further investigations of the proteomic traits of the trapezius muscle in disease, two-dimensional difference gel electrophoresis (2D-DIGE) was performed on the healthy trapezius using vastus lateralis as a reference. To obtain as much information as possible from the vast proteomic data set, both one-way ANOVA, with and without false discovery rate (FDR) correlation, and partial least square projection to latent structures with discriminant analysis (PLS-DA) were combined to compare the outcome of the analysis. RESULTS: The trapezius and vastus lateralis showed significant differences in metabolic, contractile and regulatory proteins, with different results depending on choice of statistical approach and pre-processing technique. Using the standard method, FDR correlated one-way ANOVA, 42 protein spots differed significantly in abundance between the two muscles. Complementary analysis using immunohistochemistry and western blot confirmed the results from the 2D-DIGE analysis. CONCLUSIONS: The proteomic approach used in the present study combining 2D-DIGE and multivariate modelling provided a more comprehensive comparison of the protein profiles of the human trapezius and vastus lateralis muscle, than previously possible to obtain with immunohistochemistry or SDS-PAGE alone. Although 2D-DIGE has inherent limitations it is particularly useful to comprehensively screen for important structural and metabolic proteins, and appears to be a promising tool for future studies of patients suffering from chronic work related myalgia or other muscle diseases.
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Project description:This study aimed at investigating the effect of a resistance exercise intervention on the interstitial muscle levels of pro-inflammatory cytokines in fibromyalgia (FMS) and healthy controls (CON).Twenty-four female patients with FMS (54?±?8 years) and 27 female CON (54?±?9 years) were subjected to intramuscular microdialysis of the most painful vastus lateralis muscle before and after 15 weeks of progressive resistance exercise twice per week. Baseline dialysates were sampled in the resting muscle 140 min after insertion of the microdialysis catheter. The participants then performed repetitive dynamic contractions (knee extension) for 20 min, followed by 60 min rest. Pain intensity was assessed with a 0-100 mm visual analogue scale (VAS), and fatigue was assessed with Borg's RPE throughout microdialysis. Dialysates were sampled every 20 min and analyzed with Luminex for interleukin (IL)-1?, tumor necrosis factor (TNF) alpha, IL-6, and IL-8.At both sessions and for both groups the dynamic contractions increased pain (P?<?0.012) and fatigue (P?<?0.001). The levels of TNF were lower in the FMS group than the CON group at both sessions (P?<?0.05), but none of the other cytokines differed between the groups. IL-6 and IL-8 increased after the dynamic contractions in both groups (P?<?0.010), while TNF increased only in CON (P?<?0.05) and IL-1? did not change. Overall pain intensity was reduced after the 15 weeks of resistance exercise in FMS (P?<?0.05), but there was no changes in fatigue or cytokine levels.Progressive resistance exercise for 15 weeks did not affect the interstitial levels of IL-1?, TNF, IL-6, and IL-8 in the vastus lateralis muscle of FMS patients or CON.Clinicaltrials.gov NCT01226784 , registered 21 October 2010.
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Project description:Stair descent (SD) is a common, difficult task for populations who are elderly or have orthopaedic pathologies. Joint torques of young, healthy populations during SD increase at the hip and ankle with increasing speed but not at the knee, contrasting torque patterns during gait. To better understand the sources of the knee torque pattern, we used dynamic simulations to estimate knee muscle forces and how they modulate center of mass (COM) acceleration across SD speeds (slow, self-selected, and fast) in young, healthy adults. The vastus lateralis and vastus medialis forces decreased from slow to self-selected speeds as the individual lowered to the next step. Since the vasti are primary contributors to vertical support during SD, they produced lower forces at faster speeds due to the lower need for vertical COM support observed at faster speeds. In contrast, the semimembranosus and rectus femoris forces increased across successive speeds, allowing the semimembranosus to increase acceleration downward and forward and the rectus femoris to provide more vertical support and resistance to forward progression as SD speed increased. These results demonstrate the utility of dynamic simulations to extend beyond traditional inverse dynamics analyses to gain further insight into muscle mechanisms during tasks like SD.
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Project description:Development of a method to identify age-regulated expression trends in skeletql muscles and study the underlying molecular processes of the age_associated progressive changes in skeletal muscles. See abstract from associated manuscript below : Aging is characterized by progressive tissue degeneration and it is associated with genome-wide changes of mRNA expression profiles. Therefore, it is expected that age-regulated changes in expression profiles could describe the process of aging. We have developed a robust and unbiased methodology to identify age-regulated expression trends in cross-sectional data sets from healthy donors. Our analysis in four different tissues reveals that in brain cortex and Vastus lateralis muscles, two major age-associated expression inclinations were found, one during midlife and one in elderly. In kidney medulla and cortex, however, transcriptional changes were found only in elderly. In concurrence with increased degeneration in aged tissue, the elderly-associated expression changes are predominantly downwards. Our results suggest that a non-linear trend describes the aging process, and can be the result of inclinations of gene expression at two distinct age-positions affecting different molecular and cellular processes.
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Project description:The leg of healthy volunteers was locally deconditioned using three weeks of unilateral lower limb suspension (ULLS). The extremely deconditioned legs of subjects with a spinal cord injury (SCI) were trained using eight weeks of functional electrical stimulation (FES) exercise, 2-3 times per week (total 20 sessions). These models for local (in)activity were used to study changes in gene expression level of: A) Long-term deconditioning: before FES versus before ULLS (=SCI versus control); B) Short-term deconditioning: after ULLS versus before ULLS; and C) Exercise training: after FES versus before FES.
2012-10-15 | GSE33886 | GEO
Project description:Alterations to muscle activity or loading state can induce changes in expression of myosin heavy chain (MHC). For example, sedentary individuals that initiate exercise training can induce a pronounced shift from IIx to IIa MHC. We sought to examine the regulatory response of MHC RNA in human subjects in response to exercise training. In particular, we examined how natural antisense RNA transcripts (NATs) are regulated throughout the MHC gene locus that includes MYH2 (IIa), MYH1 (IIx), MYH4 (IIb), and MYH8 (Neonatal) in vastus lateralis before and after a 5-wk training regime that consisted of a combination of aerobic and resistance types of exercise. The exercise program induced a IIx to IIa MHC shift that was associated with a corresponding increase in transcription on the antisense strand of the IIx MHC gene and a decrease in antisense transcription of the IIa MHC gene, suggesting an inhibitory mechanism mediated by NATs. We also report that the absence of expression of IIb MHC in human limb muscle is associated with the abundant expression of antisense transcript overlapping the IIb MHC coding gene, which is the opposite expression pattern as compared with that previously observed in rats. The NAT provides a possible regulatory mechanism for the suppressed expression of IIb MHC in humans. These data indicate that NATs may play a regulatory role with regard to the coordinated shifts in MHC gene expression that occur in human muscle in response to exercise training.
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Project description:This study assessed the effects of weekly disuse (dry immersion; 10 males) on the strength and aerobic performance of the ankle plantar flexors and knee extensors, mitochondrial function in permeabilized muscle fibers, and the proteomic (quantitative mass spectrometry-based analysis) and transcriptomic (RNA-sequencing) profiles of the soleus muscle and vastus lateralis muscle. Dry immersion-induced decreases in maximal voluntary contraction in both muscles were comparable and occurred without a decrease in the relative content of contractile proteins, which appears to be due to disruption of neuromuscular mechanisms rather than a decrease in muscle mass. The decrease in maximum ADP-stimulated mitochondrial respiration was also comparable in both muscles and occurred without a decrease in the relative content of mitochondrial proteins/respiratory enzymes, which appears to be associated with dysregulation of respiration. Meanwhile changes in the transcriptome were significantly more pronounced (2.5–4 times, in terms of number of differentially expressed genes) in the “slow” soleus muscle than in the vastus lateralis muscle. This suggests that longer-term disuse will lead to a change (primarily a decrease) in the relative content of a number of highly abundant proteins in Sol and an accelerated decline in the functional capacity of the ankle plantar flexors.
2024-09-08 | GSE271607 | GEO
Project description:PurposeSmall sided games (SSG) of football are an effective and efficient format to simultaneously train the physiological, technical, and tactical components of football. The duration of the recovery period between bouts of SSG will affect the physiological response to subsequent bouts. It was hypothesised that decreasing the duration of recovery periods separating serial SSG bouts would increase physiological, and perceptual responses, and decrease high speed running, and distance during SSG bouts.MethodsTwelve experienced footballers (mean ± SD; age 21 ± 3 yrs; VO2peak 64 ± 7 ml·min·kg-1; playing experience 15 ± 3 yrs) completed two SSG sessions. Each SSG consisted of 3 vs. 3 players and 6 bouts of 2 min duration, with bouts separated by either 30 s recovery (REC-30) or 120 s recovery (REC-120). Deoxygenated haemoglobin (HHb) in the vastus lateralis (VL) (using near infrared spectroscopy), heart rate (HR) and time motion descriptors (TMD) (speed and distance) were measured continuously during the SSG sessions and perceived exertion (RPE) was measured for each bout.ResultsDuring the recovery periods, in REC-30 compared to REC-120, there was a significant (p < 0.05) main effect of a higher HHb and HR. During the bouts, in REC-30 compared to REC-120, there were no significant differences in HHb, HR, RPE, or TMD, but within both REC-30 and REC-120 there were significant increases as a function of bout number in RPE.ConclusionsAlthough a four-fold increase in recovery period allowed a significant increase in the recovery of HHb and HR, this did not increase the physiological, and perceptual responses, or time motion descriptors during the bouts. These results could have been due to the regulation of effort (pacing), in these experienced players performing an exercise task to which they were well adapted.
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Project description:We investigated whether the inspiratory muscles affect maximal incremental exercise performance using a placebo-controlled, crossover design. Six cyclists each performed six incremental exercise tests. For three trials, subjects exercised with proportional assist ventilation (PAV). For the remaining three trials, subjects underwent sham respiratory muscle unloading (placebo). Inspiratory muscle pressure (P(mus)) was reduced with PAV (-35.9+/-2.3% versus placebo; P<0.05). Furthermore, V(O2) and perceptions of dyspnea and limb discomfort at submaximal exercise intensities were significantly reduced with PAV. Peak power output, however, was not different between placebo and PAV (324+/-4W versus 326+/-4W; P>0.05). Diaphragm fatigue (bilateral phrenic nerve stimulation) did not occur in placebo. In conclusion, substantially unloading the inspiratory muscles did not affect maximal incremental exercise performance. Therefore, our data do not support a role for either inspiratory muscle work or fatigue per se in the limitation of maximal incremental exercise.
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Project description:The molecular level the different muscular signature from Veteran soccer Players (VPG) vs age-matched active healthy subjects (control group, CG) was explored by using a proteomic approach. V. lateralis muscle biopsies were investigated in order to highlight the molecular mechanisms that characterize a successful aging through a lifelong football training
2023-03-11 | PXD037792 | Pride