Single-cell transcriptomics of suprachiasmatic nuclei reveal a Prokineticin-driven circadian network.

ABSTRACT: Circadian rhythms in mammals are governed by the hypothalamic suprachiasmatic nucleus (SCN), in which 20,000 clock cells are connected together into a powerful time-keeping network. In the absence of network-level cellular interactions, the SCN fails as a clock. The topology and specific roles of its distinct cell populations (nodes) that direct network functions are, however, not understood. To characterise its component cells and network structure, we conducted single-cell sequencing of SCN organotypic slices and identified eleven distinct neuronal sub-populations across circadian day and night. We defined neuropeptidergic signalling axes between these nodes, and built neuropeptide-specific network topologies. This revealed their temporal plasticity, being up-regulated in circadian day.

SUBMITTER: Morris EL 

PROVIDER: S-EPMC8521297 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature