Quantitative phosphoproteomics reveals GSK3A substrate network is involved in the cryodamage of sperm motility.

ABSTRACT: During sperm cryopreservation, the most significant phenotype of cryodamage is the decrease in sperm motility. Several proteomics studies have already been performed to search for key regulators at the protein level. However, sperm functions are known to be highly regulated by phosphorylation signaling. Here, we constructed a quantitative phosphoproteome to investigate the expression change of phosphorylated sites during sperm cryopreservation. A total of 3107 phosphorylated sites are identified and 848 of them are found to be significantly differentially expressed (DE). Bioinformatics analysis showed that the corresponding genes of these regulated sites are highly associated with sperm motility, providing a connection between the molecular basis and the phenotype of cryodamage. We then pe

SUBMITTER: Wang J 

PROVIDER: S-EPMC8521533 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature