PDZD-8 and TEX-2 regulate endosomal PI(4,5)P₂ homeostasis via lipid transport to promote embryogenesis in C. elegans.

ABSTRACT: Different types of cellular membranes have unique lipid compositions that are important for their functional identity. PI(4,5)P₂ is enriched in the plasma membrane where it contributes to local activation of key cellular events, including actomyosin contraction and cytokinesis. However, how cells prevent PI(4,5)P₂ from accumulating in intracellular membrane compartments, despite constant intermixing and exchange of lipid membranes, is poorly understood. Using the C. elegans early embryo as our model system, we show that the evolutionarily conserved lipid transfer proteins, PDZD-8 and TEX-2, act together with the PI(4,5)P₂ phosphatases, OCRL-1 and UNC-26/synaptojanin, to prevent the build-up of PI(4,5)P₂ on endosomal membranes. In the absence of t

SUBMITTER: Jeyasimman D 

PROVIDER: S-EPMC8523718 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature