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Real-time imaging of cell-surface proteins with antibody-based fluorogenic probes† † Electronic supplementary information (ESI) available: Experimental procedures, characterization of all new compounds, HRMS and NMR spectra, and supplementary figures. See DOI: 10.1039/d1sc03065e

ABSTRACT: Cell-surface proteins, working as key agents in various diseases, are the targets for around 66% of approved human drugs. A general strategy to selectively detect these proteins in a real-time manner is expected to facilitate the development of new drugs and medical diagnoses. Although brilliant successes were attained using small-molecule probes, they could cover a narrow range of targets due to the lack of suitable ligands and some of them suffer from selectivity issues. We report herein an antibody-based fluorogenic probe prepared via a two-step chemical modification under physiological conditions, to fulfill the selective recognition and wash-free imaging of membrane proteins, establishing a modular strategy with broad implications for biochemical research and for therapeutics. A modular strategy to convert commercially available antibodies into fluorogenic probes has been developed, enabling selective recognition and wash-free imaging of endogenous membrane proteins.

SUBMITTER: Wang W

PROVIDER: S-EPMC8528012 | biostudies-literature |

REPOSITORIES: biostudies-literature

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