Dataset Information

Proteome changes upon in ovo stimulation with Lactobacillus synbiotic in chicken liver.

ABSTRACT: The liver, as the main metabolic organ, plays a key role in many vital processes, including nutrient metabolism, fat digestion, blood protein synthesis, and endocrine management. As one of the immune organs, it has a remarkable ability to adequately activate the immune cells in response to metabolic signals. The anatomy of the liver ensures its close interaction with the gut so that nutrients and gut microbiota contribute to normal metabolism. In chickens, the intestinal microbiota plays an important role in supporting health and improving production parameters. The most effective method of stimulating the microbiota is to administer an appropriate bioactive compound during embryonic development. In ovo stimulation on d 12 of egg incubation involves the delivery of the substance into the air chamber. The aim of the study was to analyze the changes at the protein level after in ovo administration of the synbiotic on d 12 of egg incubation. Our study is the first to conduct a proteome analysis in liver after the administration of a Lactobacillus synbiotic in ovo. Eggs of broiler chickens were injected with a synbiotic-Lactobacillus plantarum with raffinose family oligosaccharides (RFO). On d 21 posthatching liver was collected. We performed analyses based on two-dimensional electrophoresis, matrix-assisted laser desorption/ionization (MALDI) time-of-flight, and MALDI Fourier-transform ion cyclotron resonance to obtain a global view of the hepatic proteome changes in response to in ovo injection. A representative pattern of significantly altered liver proteins was observed after stimulation with the synbiotic. A total of 16 protein spots were differentially expressed, with 5 downregulated and 11 upregulated spots. We conclude that the in ovo synbiotic treatment had the potential to accelerate the major energy-yielding metabolic pathways in the liver of adult broilers.

SUBMITTER: Dunislawska A

PROVIDER: S-EPMC8531852 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Application the innovative method which is in ovo technology provides a means of modulating the immune system at early embryonic stages. The aim of study was to determine influence of the in ovo stimulation, on d 12 of incubation, with synbiotics (synbiotic 1- L. salivarius IBB3154 + Bi2tos, Clasado Ltd. and the synbiotic 2 - L. plantarum IBB3036 + lupin RFOs) on the microstructure of duodenum, jejunum and ileum in the 1st and 42nd day of rearing.On the 1st day of chickens life, in the duodenum of both experimental groups (SYN1 and SYN2), a significantly higher and wider intestinal villi as well as a significantly larger absorbent surface of these villi were found in comparison with the Control group (P ≤ 0.01). On the 42nd day of rearing the beneficial effect of synbiotic 1 was reflected by the numerically higher villi (no statistical differences) with a larger surface (P ≤ 0.01) in the duodenum in the SYN1 group compare to the Control group. In the jejunum on the 1st day of life, in the SYN1 group, significantly higher villi than in the Control group, with a simultaneous decrease in the depth of crypts (P ≤ 0.01), and also the largest width of villi and their absorbent area (P ≤ 0.01) in comparison to the other groups were found. On the 42nd day of life, in the jejunum, an increase in the height of the villi whilst reducing the crypt depth in the SYN2 group was found (P ≤ 0.01). In turn, in the SYN1 group, there were significantly more neutral goblet cells observed compared with the control group (P ≤ 0.05). In the ileum of 1-day-old chickens, the widest villi (P ≤ 0.05) and the deepest crypts (P ≤ 0.01) were found in the SYN2 group. In the same group, there was also the least amount of neutral goblet cells in comparison to the other groups (P ≤ 0.05).We observed that synbiotic 1 and 2 beneficially affected the examined characteristics on the 1st and 42nd day of life. The obtained results allow us to conclude that the use of synbiotics significantly affect gut structure which should contribute to improvement in nutrient absorption by the gut.

Dataset Information

Proteome changes upon in ovo stimulation with Lactobacillus synbiotic in chicken liver.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets