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Sequencing of the ribosomal intergenic spacer region for strain identification of Porphyromonas gingivalis.

ABSTRACT: The ribosomal intergenic spacer regions (ISRs) of 19 laboratory strains and 30 clinical samples of Porphyromonas gingivalis were amplified by PCR and sequenced to provide a strain identifier. The ISR is a variable region of DNA located between the conserved 16S and 23S rRNA genes. This makes it an ideal locus for differentiation of strains within a species: primers specific for the conserved flanking genes were used to amplify the ISR, which was then sequenced to identify the strain. We have constructed a P. gingivalis ISR sequence database to facilitate strain identification. ISR sequence analysis provides a strain identifier that can be easily reproduced among laboratories and catalogued for unambiguous comparison.

SUBMITTER: Rumpf RW

PROVIDER: S-EPMC85328 | biostudies-literature | 1999 Aug

REPOSITORIES: biostudies-literature

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Sequencing of the ribosomal intergenic spacer region for strain identification of Porphyromonas gingivalis.

Rumpf R W RW Griffen A L AL Wen B G BG Leys E J EJ

Journal of clinical microbiology 19990801 8

The ribosomal intergenic spacer regions (ISRs) of 19 laboratory strains and 30 clinical samples of Porphyromonas gingivalis were amplified by PCR and sequenced to provide a strain identifier. The ISR is a variable region of DNA located between the conserved 16S and 23S rRNA genes. This makes it an ideal locus for differentiation of strains within a species: primers specific for the conserved flanking genes were used to amplify the ISR, which was then sequenced to identify the strain. We have con ...[more]

PMID: 10405432

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