Unknown

Dataset Information

0

Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas.


ABSTRACT: In the last few years MMTV-like nucleotide sequences were detected in some feline and canine mammary tumours. Due to the confirmed role of cats in the epidemiology of the MMTV-like virus, the aim of this study was to investigate the main pathological features of positive feline mammary carcinomas (FMCs). Twenty-four FMCs were collected at the University of Bologna, submitted to laser microdissection and analysed by nested fluorescence-PCR using primer sets specific for MMTV env sequence. For immunohistochemistry, an antibody against MMTV protein 14 (p14) was used. MMTV-like sequences were detected in three out of 24 FMCs (12.5%), one tubular carcinoma, one tubulopapillary carcinoma and one ductal carcinoma. All PCR-positive tumours were also positive for p14. Multiple nucleotide alignment has shown similarity to MMTV ranging from 98% to 100%. All the 102 examined FMCs were submitted to immunohistochemistry for molecular phenotyping. Of the nine MMTV-like positive FMCs, six were basal-like and three luminal-like. Our results demonstrate MMTV-like sequences and protein in FMCs of different geographic areas. Molecular phenotyping could contribute to understand the possible role of MMTV-like virus in FMC tumor biology.

SUBMITTER: Parisi F 

PROVIDER: S-EPMC8532932 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Pathological Features and Molecular Phenotype of MMTV Like-Positive Feline Mammary Carcinomas.

Parisi Francesca F   Muscatello Luisa Vera LV   Civita Prospero P   Lessi Francesca F   Menicagli Michele M   Millanta Francesca F   Brunetti Barbara B   Benazzi Cinzia C   Sarli Giuseppe G   Freer Giulia G   Pistello Mauro M   Mazzanti Chiara Maria CM   Poli Alessandro A  

Animals : an open access journal from MDPI 20210927 10


In the last few years MMTV-like nucleotide sequences were detected in some feline and canine mammary tumours. Due to the confirmed role of cats in the epidemiology of the MMTV-like virus, the aim of this study was to investigate the main pathological features of positive feline mammary carcinomas (FMCs). Twenty-four FMCs were collected at the University of Bologna, submitted to laser microdissection and analysed by nested fluorescence-PCR using primer sets specific for MMTV <i>env</i> sequence.  ...[more]

Similar Datasets

| S-EPMC6713336 | biostudies-literature
| S-EPMC4032816 | biostudies-literature
| S-EPMC7862120 | biostudies-literature
| S-EPMC7212821 | biostudies-literature
| S-EPMC9406662 | biostudies-literature
| S-EPMC3496133 | biostudies-literature
| S-EPMC6057629 | biostudies-literature
| S-EPMC7685478 | biostudies-literature
| S-EPMC2891995 | biostudies-literature
| S-EPMC5353915 | biostudies-literature