Dataset Information

Synaptotagmin 13 Is Highly Expressed in Estrogen Receptor-Positive Breast Cancer.

ABSTRACT:

Background

Accumulating evidence indicates tumor-promoting roles of synaptotagmin 13 (SYT13) in several cancers; however, no studies have investigated its expression in breast cancer (BC). This study aimed to clarify the significance of SYT13 in BC.

Methods

SYT13 mRNA expression levels were evaluated in BC cell lines. Polymerase chain reaction (PCR) array analysis was conducted to determine the correlation between expression levels of SYT13 and other tumor-associated genes. Then, the association of SYT13 expression levels in the clinical BC specimens with patients' clinicopathological factors was evaluated. These findings were subsequently validated using The Cancer Genome Atlas (TCGA) database.

Results

Among 13 BC cell lines, estrogen receptor (ER)-positive cells showed higher SYT13 mRNA levels than ER-negative cells. PCR array analysis revealed positive correlations between SYT13 and several oncogenes predominantly expressed in ER-positive BC, such as estrogen receptor 1, AKT serine/threonine kinase 1, and cyclin-dependent kinases 4. In 165 patients, ER-positive specimens exhibited higher SYT13 mRNA expression levels than ER-negative specimens. The TCGA database analysis confirmed that patients with ER-positive BC expressed higher SYT13 levels than ER-negative patients.

Conclusion

This study suggests that SYT13 is highly expressed in ER-positive BC cells and clinical specimens, and there is a positive association of SYT13 with the ER signaling pathways.

SUBMITTER: Ichikawa T

PROVIDER: S-EPMC8535095 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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<i>Synaptotagmin 13</i> Is Highly Expressed in Estrogen Receptor-Positive Breast Cancer.

Ichikawa Takahiro T Shibata Masahiro M Inaishi Takahiro T Soeda Ikumi I Kanda Mitsuro M Hayashi Masamichi M Takano Yuko Y Takeuchi Dai D Tsunoda Nobuyuki N Kodera Yasuhiro Y Kikumori Toyone T

Current oncology (Toronto, Ont.) 20211012 5

<h4>Background</h4>Accumulating evidence indicates tumor-promoting roles of synaptotagmin 13 (<i>SYT13</i>) in several cancers; however, no studies have investigated its expression in breast cancer (BC). This study aimed to clarify the significance of <i>SYT13</i> in BC.<h4>Methods</h4><i>SYT13</i> mRNA expression levels were evaluated in BC cell lines. Polymerase chain reaction (PCR) array analysis was conducted to determine the correlation between expression levels of <i>SYT13</i> and other tu ...[more]

PMID: 34677264

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