The variation in promoter sequences of the Akt3 gene between cow and buffalo revealed different responses against mastitis.

ABSTRACT:

Background

Serine/threonine kinase 3 (AKT3) is a protein-coding gene that is associated with several cattle immune diseases including different tumors and cancers. The objective of this study was to investigate the differences in structures and functions of AKT3 of cow and buffalo cattle.

Methods

The sequence differences of gene-coding sequence (CDS) and core promoter region of AKT3 in cow and buffalo were analyzed by using bioinformatics tools and PCR sequencing. Also, the functional analysis of promoter regulating gene expression by RT-PCR was performed using 500 Holstein cows and buffalos. And, evaluation of AKT3 inflammatory response to the lipopolysaccharide (LPS)-induced mastitis was performed between both species.

Results

The results revealed the variation in 6

SUBMITTER: Farmanullah F 

PROVIDER: S-EPMC8536807 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature