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Self-supervised pretraining for transferable quantitative phase image cell segmentation.


ABSTRACT: In this paper, a novel U-Net-based method for robust adherent cell segmentation for quantitative phase microscopy image is designed and optimised. We designed and evaluated four specific post-processing pipelines. To increase the transferability to different cell types, non-deep learning transfer with adjustable parameters is used in the post-processing step. Additionally, we proposed a self-supervised pretraining technique using nonlabelled data, which is trained to reconstruct multiple image distortions and improved the segmentation performance from 0.67 to 0.70 of object-wise intersection over union. Moreover, we publish a new dataset of manually labelled images suitable for this task together with the unlabelled data for self-supervised pretraining.

SUBMITTER: Vicar T 

PROVIDER: S-EPMC8547997 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Self-supervised pretraining for transferable quantitative phase image cell segmentation.

Vicar Tomas T   Chmelik Jiri J   Jakubicek Roman R   Chmelikova Larisa L   Gumulec Jaromir J   Balvan Jan J   Provaznik Ivo I   Kolar Radim R  

Biomedical optics express 20210924 10


In this paper, a novel U-Net-based method for robust adherent cell segmentation for quantitative phase microscopy image is designed and optimised. We designed and evaluated four specific post-processing pipelines. To increase the transferability to different cell types, non-deep learning transfer with adjustable parameters is used in the post-processing step. Additionally, we proposed a self-supervised pretraining technique using nonlabelled data, which is trained to reconstruct multiple image d  ...[more]

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