2017~2019年儿童诺如病毒相关性胃肠炎的分子流行病学研究 A molecular epidemiological study of pediatric norovirus gastroenteritis, 2017-2019
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ABSTRACT: 目的
了解2017~2019年急性胃肠炎患儿诺如病毒分子流行病学特征。 方法
回顾性收集2017~2019年于重庆医科大学附属儿童医院就诊的急性胃肠炎患儿的临床资料，采集患儿粪便标本共1 458份，提取病毒RNA，使用RT-PCR法扩增诺如病毒的衣壳蛋白VP1区，完善基因测序和基因型鉴定。 结果
1 458份粪便样本中，共检出158份阳性标本，诺如病毒阳性检出率为10.84%（158/1 458）；不同年份诺如病毒阳性检出率比较差异无统计学意义（P>0.05）。男性诺如病毒检出率为12.2%（105/860），高于女性［8.9%（53/598）］（P=0.043）。12~<18月龄患儿的诺如病毒检出率最高，为16.9%（51/301）。诺如病毒流行高峰在每年的8~10月。23份诺如病毒阳性标本合并轮状病毒感染。检出的诺如病毒主要为GII型（97.5%，154/158），GI型仅4例（2.5%，4/158）。对阳性标本进行衣壳蛋白VP1区测序发现：GII型标本中以GII.4为主，占69.6%（110/158），其中99份为GII.4 Sydney 2012，占62.7%（99/158）；其次为GII.3，占15.2%（24/158）；GII.2占10.1%（16/158）；GII.6占1.9%（3/158）；GII.17占0.6%（1/158）。GI型标本中包括GI.3，占1.3%（2/158）；GI.2、GI.5各占0.6%（1/158）。 结论
2017~2019年该院就诊的诺如病毒感染患儿以病毒为GII.4 Sydney 2012型为主，虽在全年均可存在诺如病毒感染，但8~10月份为高峰期，其间加强诺如病毒监测及重点人群防护，有助于对儿童诺如病毒腹泻进行防控。
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PROVIDER: S-EPMC8549646 | biostudies-literature |
REPOSITORIES: biostudies-literature
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