Unknown

Dataset Information

0

Structure of Venezuelan equine encephalitis virus in complex with the LDLRAD3 receptor.


ABSTRACT: LDLRAD3 is a recently defined attachment and entry receptor for Venezuelan equine encephalitis virus (VEEV)1, a New World alphavirus that causes severe neurological disease in humans. Here we present near-atomic-resolution cryo-electron microscopy reconstructions of VEEV virus-like particles alone and in a complex with the ectodomains of LDLRAD3. Domain 1 of LDLRAD3 is a low-density lipoprotein receptor type-A module that binds to VEEV by wedging into a cleft created by two adjacent E2-E1 heterodimers in one trimeric spike, and engages domains A and B of E2 and the fusion loop in E1. Atomic modelling of this interface is supported by mutagenesis and anti-VEEV antibody binding competition assays. Notably, VEEV engages LDLRAD3 in a manner that is similar to the way that arthritogenic alphaviruses bind to the structurally unrelated MXRA8 receptor, but with a much smaller interface. These studies further elucidate the structural basis of alphavirus-receptor interactions, which could inform the development of therapies to mitigate infection and disease against multiple members of this family.

SUBMITTER: Basore K 

PROVIDER: S-EPMC8550936 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| PRJNA157941 | ENA
| PRJNA438670 | ENA
| S-EPMC7769003 | biostudies-literature
2020-02-25 | GSE145815 | GEO
| S-EPMC3367636 | biostudies-literature
| S-EPMC8007291 | biostudies-literature
| S-EPMC3173789 | biostudies-literature
| S-EPMC2939143 | biostudies-literature
| S-EPMC4408325 | biostudies-literature
| S-EPMC2804100 | biostudies-literature