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Processing human lymph node samples for single-cell assays.


ABSTRACT: Most non-Hodgkin's lymphomas grow exclusively in the lymph node compartment protected by the tumor microenvironment. To better understand the cellular heterogeneity and the complex interaction between malignant and non-malignant cells, experiments with primary, patient-derived samples are often indispensable. Here, we describe a time-efficient but gentle protocol to process human lymph node samples. This protocol avoids enzymatic or mechanical stress and was optimized for the purpose of generating single-cell suspension suitable for delicate assays, such as single-cell RNA sequencing. For complete details on the use and execution of this protocol, please refer to Roider et al. (2020).

SUBMITTER: Roider T 

PROVIDER: S-EPMC8551229 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Processing human lymph node samples for single-cell assays.

Roider Tobias T   Brinkmann Berit J BJ   Dietrich Sascha S  

STAR protocols 20211021 4


Most non-Hodgkin's lymphomas grow exclusively in the lymph node compartment protected by the tumor microenvironment. To better understand the cellular heterogeneity and the complex interaction between malignant and non-malignant cells, experiments with primary, patient-derived samples are often indispensable. Here, we describe a time-efficient but gentle protocol to process human lymph node samples. This protocol avoids enzymatic or mechanical stress and was optimized for the purpose of generati  ...[more]

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2021-03-06 | GSE168358 | GEO