Unknown

Dataset Information

0

Coordination of fungal biofilm development by extracellular vesicle cargo


ABSTRACT: The fungal pathogen Candida albicans can form biofilms that protect it from drugs and the immune system. The biofilm cells release extracellular vesicles (EVs) that promote extracellular matrix formation and resistance to antifungal drugs. Here, we define functions for numerous EV cargo proteins in biofilm matrix assembly and drug resistance, as well as in fungal cell adhesion and dissemination. We use a machine-learning analysis of cargo proteomic data from mutants with EV production defects to identify 63 candidate gene products for which we construct mutant and complemented strains for study. Among these, 17 mutants display reduced biofilm matrix accumulation and antifungal drug resistance. An additional subset of 8 cargo mutants exhibit defects in adhesion and/or dispersion. Representative cargo proteins are shown to function as EV cargo through the ability of exogenous wild-type EVs to complement mutant phenotypic defects. Most functionally assigned cargo proteins have roles in two or more of the biofilm phases. Our results support that EVs provide community coordination throughout biofilm development in C. albicans. The fungal pathogen Candida albicans can release extracellular vesicles that promote biofilm formation and antifungal resistance. Here, Zarnowski et al. define functions for numerous vesicle cargo proteins in biofilm matrix assembly and drug resistance, as well as in fungal cell adhesion and dissemination.

SUBMITTER: Zarnowski R 

PROVIDER: S-EPMC8556236 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

2022-02-16 | PXD028596 | Pride
2019-11-29 | E-MTAB-7123 | biostudies-arrayexpress
2015-03-05 | E-GEOD-66488 | biostudies-arrayexpress
2020-03-01 | GSE143613 | GEO
2015-03-05 | GSE66488 | GEO
| S-ECPF-GEOD-66488 | biostudies-other
2022-10-14 | PXD035068 | Pride
| S-EPMC6198336 | biostudies-literature
| PRJEB28086 | ENA
| PRJNA601108 | ENA