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Assessment of neurodegeneration and neuronal loss in aged 5XFAD mice

ABSTRACT: Summary Neuronal loss resulting from progressive neurodegeneration is a major pathological feature of Alzheimer’s disease (AD). Here, we present a protocol to detect neurodegeneration, neuronal apoptosis, and neuronal loss in 5XFAD mouse strain, which is a well-established model for interrogating the molecular mechanism of neuronal death in AD. This protocol describes the use of the neurodegenerative marker Fluro-Jade C, cleaved caspase-3 immunofluorescent staining and Nissl staining for the analysis of neurodegeneration and neuronal loss in 5XFAD mice. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021). Graphical abstract Highlights • Aged 5XFAD mice exhibit neurodegeneration, neuroapoptosis, and neuronal loss• Analysis of neurodegeneration with the neurodegenerative marker Fluro-Jade C• Detection of apoptotic neurons by cleaved caspase-3 immunofluorescent staining• Assessment of neuronal loss by Nissl staining Neuronal loss resulting from progressive neurodegeneration is a major pathological feature of Alzheimer’s disease (AD). Here, we present a protocol to detect neurodegeneration, neuronal apoptosis, and neuronal loss in 5XFAD mouse strain, which is a well-established model for interrogating the molecular mechanism of neuronal death in AD. This protocol describes the use of the neurodegenerative marker Fluro-Jade C, cleaved caspase-3 immunofluorescent staining, and Nissl staining for the analysis of neurodegeneration and neuronal loss in 5XFAD mice.

SUBMITTER: Zhang L

PROVIDER: S-EPMC8556527 | biostudies-literature |

REPOSITORIES: biostudies-literature

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