Isolation and characterization of neural stem/progenitor cells in the subventricular zone of the naked mole-rat brain.

ABSTRACT:

Background

The naked mole-rat (NMR) is the longest-lived rodent with a maximum lifespan of more than 37 years and shows a negligible senescence phenotype, suggesting that tissue stem cells of NMRs are highly capable of maintaining homeostasis. However, the properties of NMR tissue stem cells, including neural stem cells (NSCs), are largely unclear.

Methods

Neural stem/progenitor cells (NS/PCs) were isolated from the subventricular zone of the neonate NMR brain (NMR-NS/PCs) and cultured in neurosphere and adherent culture conditions. Expression of NSC markers and markers of neurons, astrocytes, and oligodendrocytes was analyzed by immunocytochemistry. In adherent culture conditions, the proliferation rate and cell cycle of NMR-NS/PCs were assessed and compared with those of N

SUBMITTER: Yamamura Y 

PROVIDER: S-EPMC8559411 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature